Development of 3D human intestinal equivalents for substance testing in microliter-scale on a multi-organ-chip
نویسندگان
چکیده
Background Robust and reliable dynamic bioreactors for long term maintenance of various tissues at milliliter-scale on the basis of a biological, vascularized matrix (BioVaSc) have been developed at the Fraunhofer IGB in Stuttgart, Germany. As an intestinal in vitro equivalent, seeding of the matrix with CaCo-2 cells yielded in the self-assembly of a microenvironment with the typical histological appearance of villus-like structure and morphology [1]. We modified this matrix (BioVaSc) cell (CaCo-2) system to some extent with the aim to develop 3D intestinal equivalents for systemic preclinical testing of orally applied drug candidates in microliter-scale on a human Multi-Organ-Chip (MOC), which consists of different organ equivalents important for ADMET (adsorption, distribution, metabolism, excretion, toxicity) testing.
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